RaNA-seq Tutorial: Web-Based RNA-Seq Data Analysis
What is RaNA-seq?
RaNA-seq is a tool that lets you perform RNA-Seq data analysis directly in your web browser. Starting from FASTQ files, you can carry out everything from extracting differentially expressed genes to functional analysis. This page walks you through how to use RaNA-seq. Although RaNA-seq is free, the server can be unstable at times and certain operations may not be intuitive. If you are looking for an alternative, please also consider the RNA-Seq Data Analysis Tool offered by our company.
Login
Click "Analysis" in the menu to bring up the user registration screen shown below. You can also get started without logging in by selecting "Anonymous access."
Uploading Samples
After logging in, the following screen will appear. Select your reference sequence under "Reference genome assembly," choose single-end or paired-end under "Sequencing type," and then upload your FASTQ files via "Select your FASTQ Files." Depending on the file size, the upload may take some time.
Quality Control
Once the upload is complete, click "Quality Control" on the following screen to view the QC results.
In addition to Boxplots, you can also view Barplots, Heatmaps, PCA plots, and other visualizations.
Analysis
Once the upload is complete, clicking "Analysis" will bring up the settings screen shown below.
Click "Results" to see the following screen. After a short wait, the individual results will become available for viewing.
RNA-Seq Data Analysis Software
This is an RNA-Seq Data Analysis Software recommended for those who:
✔︎ Seeking to avoid outsourcing or collaboration for RNA-Seq data analysis.
✔︎ Lacking time to learn RNA-Seq data analysis.
✔︎ Frustrated by the complexity of existing tools.
Users can perform gene expression quantification, identification of differentially expressed genes, gene ontology(GO) analysis, pathway analysis, as well as drawing volcano plots, MA plots, and heatmaps.
About the Author
BxINFO LLC
A research support company specializing in bioinformatics.
We provide tools and information to support life science research, with a focus on RNA-Seq analysis.