How to Use RaNA-seq: A Guide to RNA-Seq Data Analysis
What is RaNA-seq?
RaNA-seq is a tool that allows you to perform RNA-Seq data analysis directly in your web browser. Starting from FASTQ files, you can perform tasks ranging from extracting differentially expressed genes to functional analysis. This page introduces how to use RaNA-seq. While RaNA-seq is free to use, the server can sometimes be unstable and some operations may be counterintuitive. If you are looking for an alternative, please also consider the RNA-Seq Data Analysis Software provided by our company.
Login
Clicking "Analysis" in the menu will display the user registration screen shown below. You can also start without logging in by selecting "Anonymous access."
Uploading Samples
Once logged in, the following screen will appear. After selecting the reference sequence in "Reference genome assembly" and choosing between single-end or paired-end for the "Sequencing type," proceed to upload your FASTQ files via "Select your FASTQ Files." Depending on the file size, the upload process may take some time.
Quality Control
After the upload is complete, click "Quality Control" on the following screen to check the QC results.
In addition to Boxplots, you can also view Barplots, Heatmaps, PCA plots, and more.
Analysis
After the upload is complete, clicking "Analysis" will display the settings screen shown below.
Clicking "Results" will display the following screen, and after a short wait, each result will become available for viewing.
RNA-Seq Data Analysis Software
This is an RNA-Seq Data Analysis Software recommended for those who:
✔︎ Seeking to avoid outsourcing or collaboration for RNA-Seq data analysis.
✔︎ Lacking time to learn RNA-Seq data analysis.
✔︎ Frustrated by the complexity of existing tools.
Users can perform gene expression quantification, identification of differentially expressed genes, gene ontology(GO) analysis, pathway analysis, as well as drawing volcano plots, MA plots, and heatmaps.
About the Author
BxINFO LLC
A research support company specializing in bioinformatics.
We provide tools and information to support life science research, with a focus on RNA-Seq analysis.