Tm Calculator
This tool calculates the Tm using the nearest-neighbor method based on the primer's nucleotide sequence.
Results
| Primer Sequence | - |
|---|---|
| Reverse Complement | - |
| Length | - bases |
| GC Content | - % |
| Tm | - ℃ |
Note
- This tool calculates the Tm using the nearest-neighbor method based on the primer's nucleotide sequence.
- Please consider this as one of the parameters when designing primers.
- Tm is the temperature at which 50% of the double-stranded DNA dissociates into single-stranded DNA.
- It is said that the reaction works best when the annealing temperature for PCR is set at a temperature about 5℃ lower than the Tm
- The annealing temperature for PCR is typically set between 55℃ to 60℃.
- The values of ΔH and ΔS are taken from the reference.
- By checking the box for “Multiple Sequences“, you can calculate the Tm of multiple primer sequences at once.