Tm Calculator – Primer Melting Temperature (Nearest-Neighbor Method)
This tool calculates the Tm based on the primer sequence you enter.
Results
| Primer Sequence | - |
|---|---|
| Reverse Complement | - |
| Length | - bases |
| GC Content | - % |
| Tm | - ℃ |
Note
- This tool calculates the Tm based on the primer sequence you enter.
- Please consider this as one of the parameters when designing primers.
- Tm is the temperature at which 50% of the double-stranded DNA dissociates into single-stranded DNA.
- It is said that the reaction works best when the annealing temperature for PCR is set at a temperature about 5℃ lower than the Tm
- The annealing temperature for PCR is typically set between 55℃ to 60℃.
- The Tm is calculated using the nearest-neighbor method.
- The values of ΔH and ΔS are taken from the reference.
- By checking the box for “Multiple Sequences“, you can calculate the Tm of multiple primer sequences at once.
- If you choose “Find primer sequences with a target Tm“, every subsequence of the specified length is extracted from the target sequence and listed with its Tm.
References
RNA-Seq Data Analysis Software
This is an RNA-Seq Data Analysis Software recommended for those who:
✔︎ Seeking to avoid outsourcing or collaboration for RNA-Seq data analysis.
✔︎ Lacking time to learn RNA-Seq data analysis.
✔︎ Frustrated by the complexity of existing tools.
Users can perform gene expression quantification, identification of differentially expressed genes, gene ontology(GO) analysis, pathway analysis, as well as drawing volcano plots, MA plots, and heatmaps.