Tm Calculator
This tool calculates the Tm using the nearest-neighbor method based on the primer's nucleotide sequence.
Results
| Primer Sequence | - |
|---|---|
| Reverse Complement | - |
| Length | - bases |
| GC Content | - % |
| Tm | - ℃ |
Note
- This tool calculates the Tm using the nearest-neighbor method based on the primer's nucleotide sequence.
- Please consider this as one of the parameters when designing primers.
- Tm is the temperature at which 50% of the double-stranded DNA dissociates into single-stranded DNA.
- It is said that the reaction works best when the annealing temperature for PCR is set at a temperature about 5℃ lower than the Tm
- The annealing temperature for PCR is typically set between 55℃ to 60℃.
- The values of ΔH and ΔS are taken from the reference.
- By checking the box for “Multiple Sequences“, you can calculate the Tm of multiple primer sequences at once.
References
RNA-seq Data Analysis Software – No Bioinformatician Needed
With our RNA-Seq data analysis software, you won't need to outsource or rely on collaborators. You can start analyzing the data yourself right away, without the need for high-spec computers or knowledge of Linux commands.
Users can perform gene expression quantification, identification of differentially expressed genes, gene ontology(GO) analysis, pathway analysis, as well as drawing volcano plots, MA plots, and heatmaps.