Calculate Agarose Amount for Gel Electrophoresis – Recommended Concentrations

This tool helps you with calculations for agarose gel electrophoresis.

• Specify the DNA size to see the recommended gel concentration, and calculate the required amount of agarose based on buffer volume.
• If the gel concentration is already determined, calculate the required amount of agarose based on buffer volume.
• If the agarose weight and buffer volume are known, calculate the gel concentration.

Description

• Agarose amount (by DNA size): Calculate the required amount of agarose from the target DNA size and buffer volume.
  • Based on Thermo Fisher Scientific recommendations, the gel concentration is automatically selected according to DNA size.
  • Formula: Agarose (g) = Recommended concentration × Volume (mL) ÷ 100
• Agarose amount (by gel concentration): Calculate the required amount of agarose from the specified gel concentration and buffer volume.
  • Formula: Agarose (g) = Concentration (% (w/v)) × Volume (mL) ÷ 100
• Gel concentration: Calculate the gel concentration from the agarose weight and buffer volume.
  • Formula: Concentration (% (w/v)) = Agarose (g) ÷ Volume (mL) × 100

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